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Research on plant oxygen-sensing mechanisms has demonstrated that cysteine oxidase genes(PCOs) serve as critical oxygen sensors, modulating oxygen-responsive signaling pathways to support the normal growth and development of plant under stress conditions, thereby conferring a significant survival advantage. The coding sequence(CDS) of the peanut AhPCO gene was successfully cloned in this study, which spans 843 bp and encodes a protein consisting of 281 amino acids with a theoretical isoelectric point(pI) of 6.06. The protein exhibits hydrophilicity, instability, and lack of signal peptide, and is primarily localized in the nucleus and cytoplasm. Phylogenetic analysis revealed a close evolutionary relationship between the AhPCO protein and AtPCO1 from Arabidopsis thaliana. Analysis of the AhPCO promoter region suggested that the transcription may be induced by abscisic acid(ABA) and anaerobic conditions. The qPCR results showed that AhPCO exhibits tissue-specific expression, with the expression levels in different peanut tissues as the following order: seed > stem > root > leaf > flower. Stress treatment experiments showed that, compared with the control group, AhPCO expression was significantly upregulated after 2 h of high-temperature treatment and 12 h of salt stress. In contrast, AhPCO expression decreased significantly after 15 d of drought stress, and increased sharply while following 1 d of rehydration. Although the expression gradually declined over the subsequent 2 to 4 d, it remained higher than that in the control. The AhPCO gene is significantly regulated by various abiotic stresses, including high temperature, salinity, drought, and rehydration, and exhibits tissue-specific expression patterns. It may play a crucial regulatory role in the stress response and recovery processes of peanuts under environmental stress conditions. This study lays a theoretical foundation for elucidating the function of AhPCO gene and provides potential genetic resources for breeding stress-tolerant, high-quality peanut varieties.
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Basic Information:
DOI：10.14001/j.issn.1002-4093.2026.01.001
China Classification Code:S565.2
Citation Information:
[1]TANG Wei,ZHU Zhenquan,RAN Haixia ,et al.Cloning and Expression Analysis of Cysteine Oxidase Gene in Peanut[J].Journal of Peanut Science,2026,55(01):1-11.DOI:10.14001/j.issn.1002-4093.2026.01.001.
Fund Information:
国家自然科学基金项目(32260441); 贵州省科学技术厅基金项目(ZK2021-127); 贵州省大学生创新创业训练项目(ZHCX2023023,ZHCX2022018); 贵州省高等学校教学内容和课程体系改革项目(2023169)