TANG Yueyi
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To explore the molecular mechanism of AhRabF1 gene regulating peanut resistance to early leaf spot disease, the peanut AhRabF1 gene was cloned in this study, and its complete coding region(CDS) was obtained, consisting of 603 base pairs(bp), encoding 200 amino acids with a protein molecular weight(MW) of 21.0 kDa and an isoelectric point(pI) of 6.16. Notably, no transmembrane structure was identified. Subcellular localization prediction results showed that the protein is hydrophilic and predominantly located in the cytoplasm. The expression pattern of this gene was analyzed using qRT-PCR, revealing that the gene exhibited the highest expression abundance in leaves. Furthermore, its expression level was higher in disease-resistant peanut materials than that in susceptible ones. Following infection by the pathogen, the expression of AhRabF1 initially increased and then decreased, reaching peak on the 9th day of post-inoculation(DPI). To further elucidate the role of AhRabF1 in response to early leaf spot disease, virus-induced gene silencing(VIGS) was employed to inhibit the gene expression in the disease-resistant material U1. The results indicated that the expression level of AhRabF1 in the leaves of gene-silenced plants was lower than that in the control group, ranging from 0.26 to 0.82 times the expression level of the control. Moreover, following pathogen inoculation, the disease resistance of the gene-silenced plants was significantly diminished. Therefore, it is hypothesized that the AhRabF1 gene may play role in peanut resistance to early leaf spot disease.
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Basic Information:
DOI：10.14001/j.issn.1002-4093.2026.02.002
China Classification Code:S565.2
Citation Information:
[1]HU Weidong,GUAN Shuyan,ZHU Lifei ,et al.Cloning and Preliminary Functional Analysis of AhRabF1 in Peanut[J].Journal of Peanut Science,2026,55(02):161-170.DOI:10.14001/j.issn.1002-4093.2026.02.002.
Fund Information:
山东省重点研发计划项目(2024TZXD057);山东省重点研发计划(农业良种工程)(2022LZGC007); 山东省自然科学基金(ZR2021MC040); 青岛市科技惠民示范专项(24-1-8-xdny-5-nsh); 新疆维吾尔自治区重大科技专项(2022A02008); 山东省农业科学院农业科技创新工程(CXGC2022F33)
2026-02-13
2026-02-13
2026-02-13