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Peanut(Arachis hypogaea L.) is an important oilseed and economic crop, yet its tolerance to low temperature is relatively limited. Low-temperature stress severely impairs seedling survival, growth, development, and final fruit yield. Thus, the identification of cold-tolerance genes is crucial for safeguarding production and facilitating breeding improvement. In this study, transcriptome sequencing was performed with the cultivar Huayu6316 to analyze gene expression profiles at 0 h and 2 h under low-temperature stress. A total of 3,806 differentially expressed genes(DEGs) were identified in response to the stress. The majority of these DEGs were significantly enriched in conserved pathways, including photosynthesis, active oxygen metabolism, carbohydrate metabolism, hormone signal transduction, and notably, the circadian clock pathway. Importantly, the involvement of the circadian clock pathway in the early response to low-temperature stress was revealed in this study. The rapid upregulation of its core genes suggested that the circadian system was deeply engaged in low-temperature signal perception and the coordinated construction of regulatory networks, offering a new theoretical perspective for understanding the cold-tolerance mechanisms in crops. Furthermore, quantitative real-time PCR(qRT-PCR) validation of 21 key DEGs from various pathways led to the identification of several promising cold-tolerance candidate genes, including Ah14g358300, Ah14g142600, Ah09g008300, Ah03g413700, and Ah09g196000. This study provided a theoretical foundation for further exploration of cold-tolerance genes, elucidation of their underlying mechanisms, and breeding improvement.
[1] DING Y L,SHI Y T,YANG S H.Regulatory networks underlying plant responses and adaptation to cold stress [J].Annual Review of Genetics,2024,58(1):43-65.
[2] BITTNER A,GRIEBEL T,VAN BUER J,et al.Determining the ROS and the antioxidant status of leaves during cold acclimation [J].Methods in Molecular Biology,2020,2156:241-254.
[3] YU H,KONG X,HUANG H,et al.STCH4/REIL2 confers cold stress tolerance in Arabidopsis by promoting rRNA processing and CBF protein translation [J].Cell Reports,2020,30(1):229-242.
[4] LIU Y,CAI Y J,LI Y Z,et al.Dynamic changes in the transcriptome landscape of Arabidopsis thaliana in response to cold stress [J].Frontiers in Plant Science,2022,13:983460.DOI:10.3389/fpls.2022.983460.
[5] LI X J,GARCíA-CAPARROS P,SUN Y D,et al.Integrating Meta-QTL mapping and RNA-seq analysis identifies candidate genes for cold tolerance at rice seedling stage [J].BMC Plant Biology,2026,26:395.DOI:10.1186/s12870-026-08224-3.
[6] MAZURIER M,DROUAUD J,BAHRMAN N,et al.Integrated sRNA-seq and RNA-seq analyses reveal a microRNA regulation network involved in cold response in Pisum sativum L.[J].Genes,2022,13:1119.DOI:10.3390/genes13071119.
[7] LIU L,SI L,ZHANG L H,et al.Metabolomics and transcriptomics analysis revealed the response mechanism of alfalfa to combined cold and saline-alkali stress [J].Plant Journal,2024,119(4):1900-1919.
[8] CROSATTI C,RIZZA F,BADECK F W,et al.Harden the chloroplast to protect the plant [J].Physiologia Plantarum,2013,147(1):55-63.
[9] 周桦楠，潘家荃，刘冠求，等.甘薯响应冷胁迫的转录组分析[J].植物遗传资源学报，2022,23(4):1202-1212.
[10] 邱彤.小麦苗期低温生理响应及转录组分析[D].合肥：安徽农业大学，2021.
[11] AN Z X,YANG Z H,ZHOU Y,et al.OsJRL negatively regulates rice cold tolerance via interfering phenylalanine metabolism and flavonoid biosynthesis [J].Plant Cell and Environment,2024,47(11):4071-4085.
[12] 张鑫苗，伍国强，魏明.MAPK在植物响应逆境胁迫中的作用[J].草业学报，2024,33(1):182-197.
[13] 韩尧，李燕萍.红曲霉ABC转运蛋白基因家族的鉴定与生物信息学分析[J].南昌大学学报，2023,47(2):148-156.
[14] SUH J Y,KIM W T.Arabidopsis RING E3 ubiquitin ligase AtATL80 is negatively involved inphosphate mobilization and cold stress response in sufficient phosphate growth conditions [J].Biochemical and Biophysical Research Communications,2015,463(4):793-799.
[15] 温晴，梁鑫雅，王彬彬，等.低温胁迫北柴胡WRKY基因的转录组水平鉴定与特征分析[J/OL].分子植物育种，(2025-10-20)[2026-02-02].https://link.cnki.net/urlid/46.1068.s.20251020.0945.002.
[16] 万慧兰，吴华英，曾丹，等.蔗茅耐寒相关基因EfWRKY51克隆分析及功能验证[J].作物学报，2025,51(8):2048-2059.
[17] 刘小玉，周丽霞，曹红星，等.油棕幼苗耐寒性生理指标分析及评价体系构建[J/OL].分子植物育种，(2023-11-15)[2026-02-02].https://link.cnki.net/urlid/46.1068.s.20231115.1402.016.
[18] FU D Y,SONG Y,WU S F,et al.Regulation of alternative splicing by CBF-mediated protein condensation in plant response to cold stress [J].Nature Plants,2025,11(3):505-517.
[19] DE LEONE M J,YANOVSKY M J.The circadian clock and thermal regulation in plants:novel insights into the role of positive circadian clock regulators in temperature responses [J].Journal of Experimental Botany,2024,75(10):2809-2818.
[20] LIU C L,HE L L,JIANG M,et al.Genome-wide analysis of Zoysia japonica REVEILLE family identifies ZjRVE8-1 as a positive cold-tolerance regulator [J].Plant Cell Reports,2025,45(1):14.DOI:10.1007/s00299-025-03700-w.
[21] XU L,YANG L J,LI A P,et al.An AP2/ERF transcription factor confers chilling tolerance in rice [J].Science Advances,2024,10(35):eado4788.DOI:10.1126/sciadv.ado4788.
[22] AGURLA S,GAHIR S,MUNEMASA S,et al.Mechanism of stomatal closure in plants exposed to drought and cold stress [J].Advances in Experimental Medicine and Biology,2018,1081:215-232.
[23] 张慧玉，岳丹斐，潘晓娇，等.5-羟色胺在红树植物秋茄幼苗抗寒中的作用[J].应用生态学报，2023,34(5):1263-1271.
[24] CHATER C C C,OLIVER J,CASSON S,et al.Putting the brakes on:abscisic acid as a central environmental regulator of stomatal development [J].New Phytologist,2014,202(2):376-391.
[25] 董敬超，于树涛，王虹，等.花生发芽期与出苗期耐低温种质的筛选和鉴定 [J].农业科技通讯，2025(5):111-118.
[26] WANG X,LIU Y,HAN Z K,et al.Integrated transcriptomics and metabolomics analysis reveal key metabolism pathways contributing to cold tolerance in peanut [J].Frontiers in Plant Science,2021,12:752474.DOI:10.3389/fpls.2021.752474.
[27] 张高华，于树涛，王鹤，等.高油酸花生发芽期低温胁迫转录组及差异表达基因分析[J].遗传，2019,41(11):1050-1059,1073.
[28] JIANG C J,ZHANG H,REN J Y,et al.Comparative transcriptome-based mining and expression profiling of transcription factors related to cold tolerance in peanut [J].International Journal of Molecular Sciences,2020,21(6):1921.DOI:10.3390/ijms21061921.
[29] WANG X,LIU Y,OUYANG L,et al.Full-length transcriptome sequencing provides insights into alternative splicing under cold stress in peanut [J].Frontiers in Plant Science,2024,15:1362277.DOI:10.3389/fpls.2024.1362277.
[30] RAZA A,SHARIF Y,CHEN K,et al.Genome-wide characterization of ascorbate peroxidase gene family in peanut (Arachis hypogea L.) revealed their crucial role in growth and multiple stress tolerance [J].Frontiers in Plant Science,2022,13:962182.DOI:10.3389/fpls.2022.962182.
[31] LOVE M I,HUBER W,ANDERS S.Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2 [J].Genome Biology,2014,15(12):550.DOI:10.1186/s13059-014-0550-8.
[32] LIVAK K J,SCHMITTGEN T D.Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method [J].Methods,2001,25(4):402-408.
[33] CHEN S.fastp 1.0:An ultra-fast all-round tool for FASTQ data quality control and preprocessing [J].iMeta,2025,4(5):e70078.DOI:10.1002/imt2.70078.
[34] PUTRI G H,ANDERS S,PYL P T,et al.Analysing high-throughput sequencing data in Python with HTSeq 2.0 [J].Bioinformatics,2022,38(10):2943-2945.
[35] 宋兆锋，陈小姝，李美君，等.低温胁迫下赤霉素对花生萌发特性的影响及转录组分析[J].花生学报，2023,52(3):8-19.
[36] 林靖童，王金刚，魏殿文，等.基于转录组测序的蓝果忍冬水分胁迫关键抗逆基因挖掘[J].经济林研究，2025,43(1):17-28.
[37] 卢文田，曹凤鸣，周静文，等.应用转录组测序技术分析干旱胁迫下大豆落花落荚的调控基因 [J].东北农业大学学报，2024,55(8):14-27.
[38] WU N,FENG Y Q,JIANG T,et al.Genome-wide study and expression analysis of soybean ERF transcription factors and over expression of GmERF205 enhances drought resistance in soybean [J].BMC Genomics,2025,26(1):726.DOI:10.1186/s12864-025-11829-x.
[39] VERMA P K,BUROW M D,SIMPSON C E,et al.Transcriptomic insights into drought response in wild Arachis relatives A.dardani and A.ipaënsis [J].BMC Plant Biology,2025,26(1):103.DOI:10.1186/s12870-025-07799-7.
[40] HUANG L T,LIU C Y,LI L,et al.Genome-wide identification of bZIP transcription factors in faba bean based on transcriptome analysis and investigation of their function in drought response [J].Plants (Basel),2023,12(17):3043.DOI:10.3390/plants12173041.
[41] CHAUDHARY C,KANWAR M,SIROHI P,et al.Transcriptome mapping of unfertilized pea flowers under high temperature:insights into gene regulatory networks genomics resources [J].Plant Physiology and Biochemistry,2025,228:110268.DOI:10.1016/j.plaphy.2025.110268.
[42] 何梅，张佳蕾，范士凯，等.花生抗旱基因AhCPK8及其互作蛋白GAPDH的克隆及分子鉴定[J].作物学报，2025,51(10):2713-2726.
[43] 徐扬，张智猛，秦斐斐，等.基于多组学方法探究花生花针期响应盐胁迫的机制[J].花生学报，2025,54(3):225-234.
[44] SHAH S S T H,XIE C X,DENG M,et al.Dual SA-mediated and SA-independent mechanisms drive Sphingomonas paucimobilis-induced cold tolerance in Arabidopsis [J].Journal of Applied Microbiology,2025,136(12):lxaf301.DOI:10.1093/jambio/lxaf301.
[45] LI J,GUO H F,LOU Q J,et al.Natural variation of indels in the CTB3 promoter confers cold tolerance in japonica rice [J].Nature Communications,2025,16(1):1613.DOI:10.1038/s41467-025-56992-7.
[46] ZHAO X T,ALI A,FENG C L,et al.Genome-wide analysis of the sugarcane SUT gene family reveals ShSUT4 as a key regulator of abiotic stress responses [J].Theoretical and Applied Genetics,2026,139(1):26.DOI:10.1007/s00122-025-05138-w.
[47] 王玲宝，魏姗姗，赵静雅，等.拟南芥生物钟调控ABI5近日节律表达的研究[J].农业与技术，2025,45(24):40-43.
[48] TEIGE M,SCHEIKL E,EULGEM T,et al.The MKK2 pathway mediates cold and salt stress signaling in Arabidopsis [J].Molecular Cell,2004,15(1):141-152.
[49] SORKIN M L,TZENG S C,KING S,et al.COLD REGULATED GENE 27 and 28 antagonize the transcriptional activity of the RVE8/LNK1/LNK2 circadian complex [J].Plant Physiology,2023,192(3):2436-2456.
[50] XIA C X,GONG Y S,CHONG K,et al.Phosphatase OsPP2C27 directly dephosphorylates OsMAPK3 and OsbHLH002 to negatively regulate cold tolerance in rice [J].Plant Cell and Environment,2021,44(2):491-505.
[51] ZHAO Y W,WU Z Z,LI J Y,et al.The key role of cytochrome P450s in the biosynthesis of plant derived natural products [J].Plant Physiology and Biochemistry,2025,222:109695.DOI:10.1016/j.plaphy.2025.109695.
[52] ZHAO L Y,YANG T Y,XING C H,et al.The β-amylase PbrBAM3 from pear (Pyrus betulaefolia) regulates soluble sugar accumulation and ROS homeostasis in response to cold stress [J].Plant Science,2019,287:110184.DOI:10.1016/j.plantsci.2019.110184.
[53] CHEN S,HUANG H A,CHEN J H,et al.SgRVE6,a LHY-CCA1-like transcription factor from fine-stem stylo,upregulates NB-LRR gene expression and enhances cold tolerance in tobacco [J].Frontiers in Plant Science,2020,11:1276.DOI:10.3389/fpls.2020.01276.
[54] MEISSNER M,ORSINI E,RUSCHHAUPT M,et al.Mapping quantitative trait loci for freezing tolerance in a recombinant inbred line population of Arabidopsis thaliana accessions Tenela and C24 reveals REVEILLE1as negative regulator of cold acclimation [J].Plant Cell and Environment,2013,36(7):1256-1267.
[55] ZHOU P,LI X S,LIU X J,et al.Transcriptome profiling of Malus sieversii under freezing stress after being cold-acclimated [J].BMC Genomics,2021,22:681.DOI:10.1186/s12864-021-07998-0.
[56] GUSAIN S,JOSHI S,JOSHI R.Sensing,signalling,and regulatory mechanism of cold-stress tolerance in plants [J].Plant Physiology and Biochemistry,2023,197:107646.DOI:10.1016/j.plaphy.2023.107646.
[57] ZHANG Q Y,LI Y,CAO K,et al.Transcriptome and proteome depth analysis indicate ABA,MAPK cascade and Ca2+ signaling co-regulate cold tolerance in Rhododendron chrysanthum Pall [J].Frontiers in Plant Science,2023,14:1146663.DOI:10.3389/fpls.2023.1146663.
[58] XING K F,ZHANG J,XIE H X,et al.Identification and analysis of MAPK cascade gene families of Camellia oleifera and their roles in response to cold stress [J].Molecular Biology Reports,2024,51:602.DOI:10.1007/s11033-024-09551-0.
[59] BORO P,SULTANA A,MANDAL K,et al.Interplay between glutathione and mitogen-activated protein kinase 3 via transcription factor WRKY40 under combined osmotic and cold stress in Arabidopsis [J].Journal of Plant Physiology,2022,271:153664.DOI:10.1016/j.jplph.2022.153664.
Basic Information:
DOI：10.14001/j.issn.1002-4093.2026.02.001
China Classification Code:S565.2
Citation Information:
[1]LIU Yulong,HU Rong,YUAN Cuiling ,et al.Transcriptome Analysis of Response to Low Temperature Stress in Peanut[J].Journal of Peanut Science,2026,55(02):145-160+248.DOI:10.14001/j.issn.1002-4093.2026.02.001.
Fund Information:
山东省重点研发计划项目(2025LZGC019); 山东省花生产业技术体系(SDAIT-04-01); 辽宁省种质资源创新藏粮于技专项计划(2023JH1/10200002); 泰安市农业良种项目(2024NYLZ14)
2026-04-21
2026-04-21
2026-04-21